March 17: Introduction to CryoSPARC for Cryo-EM data using HCC

Pre-Register here: https://hcc.unl.edu/introduction-cryosparc-cryo-em-data-using-hcc

This workshop will give participants a hands-on experience on running CryoSPARC and processing Cryo-EM data on HCC resources. Participants will learn how to access and launch CryoSPARC on HCC Swan, and how to configure CryoSPARC database and project directories. Participants will also learn how to use CPU and GPU resources for the various CryoSPARC jobs. Part of this training will utilize HCC's new National Science Foundation-funded GPU resource named PLUMAGE (Promoting Learning Using Mixed Advanced GPU Environments). During the workshop, we will process the EMPIAR-10059 dataset, which contains TRPV1 in complex with DkTx and RTX in lipid nanodiscs.

Topics to include:

• Introduction to HCC resources

• Access CryoSPARC on HCC Swan

• Navigate CryoSPARC database and project directories

• Launch CryoSPARC on HCC Swan

• Complete hands-on tutorial of Cryo-EM data processing

Participants are required to have a valid individual academic CryoSPARC license ID and are recommended to sign up for an HCC account (https://hcc.unl.edu/new-user-request). Temporary HCC accounts will be available during the workshop if needed.

Pre-Register for the training:https://hcc.unl.edu/introduction-cryosparc-cryo-em-data-using-hcc

When: March 17th, 9:30 AM - 4:30 PM CDT

Where:UNL City Campus, Brace Lab 105 and via Zoom

Cost: $50 for NU Participants
* NU Participants include UNL, UNO, UNMC, and UNK
For all other participants, please visit the workshop webpage below.

Schedule available on workshop webpage: https://hcc.unl.edu/introduction-cryosparc-cryo-em-data-using-hcc.